Production and identification of intricate bioactive oligosaccharides from Nyctanthesarbor-tristis leaves by a combination of enzymatic, HPAEC and MALDI-TOF-MS techniques
By: Imran Ali
.
Contributor(s): Mukherjee, Shuvam.
Publisher: New Delhi CSIR 2021Edition: Vol.60(B), Nov.Description: 1471-1477p.Subject(s): GENERAL CHEMISTRYOnline resources: Click here
In:
Indian journal of chemistry (Section B)Summary: Non-digestible oligosaccharides are associated with positive health outcomes; however, hemicellulosic oligosaccharides
of N. arbor-tristis have not been studied. Herein, we report production of hemicellulosic oligosaccharides from its leaves
and their identification. A series of complex neutral oligosaccharides ( 1-16), produced by endo-(1,4)--D-glucanase
digestion of the alkali extracted xyloglucan, have been identified by chemical, HPAE chromatographic and MALDI-mass
spectrometric techniques. Additionally, xylan enriched hemicellulose-A fraction upon endo-(1,4)--D-xylanase digestion
yield several acidic and neutral xylo-oligosaccharides ( 17-24), each containing a backbone of -(1,4)-D-Xylp units
substituted at position 2 with a single stub of D-Xylp or 4-O-Me-D-GlcpA residues. The structural diversity and high water
solubility of these enzyme-generated oligosaccharides suggested promising health implications.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2022-0171 |
Non-digestible oligosaccharides are associated with positive health outcomes; however, hemicellulosic oligosaccharides
of N. arbor-tristis have not been studied. Herein, we report production of hemicellulosic oligosaccharides from its leaves
and their identification. A series of complex neutral oligosaccharides ( 1-16), produced by endo-(1,4)--D-glucanase
digestion of the alkali extracted xyloglucan, have been identified by chemical, HPAE chromatographic and MALDI-mass
spectrometric techniques. Additionally, xylan enriched hemicellulose-A fraction upon endo-(1,4)--D-xylanase digestion
yield several acidic and neutral xylo-oligosaccharides ( 17-24), each containing a backbone of -(1,4)-D-Xylp units
substituted at position 2 with a single stub of D-Xylp or 4-O-Me-D-GlcpA residues. The structural diversity and high water
solubility of these enzyme-generated oligosaccharides suggested promising health implications.
Click on an image to view it in the image viewer
There are no comments for this item.